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Η συγκόλληση που εμφανίζεται είναι αποτέλεσμα σύνδεσης (cross-linking) των ερυθρών αιμοσφαιρίων μέσω αντισωμάτων. Τα anti-A και anti-B περιέχουν κυρίως IgM αντισώματα. Τα IgM έχουν πενταμερή δομή και μπορούν να συνδέσουν πολλά κύτταρα ταυτόχρονα, δημιουργώντας ένα «δίκτυο» (lattice formation). Γι’ αυτό η αιμοσυγκόλληση είναι άμεση και ορατή. Το anti-D (για τον Rhesus) αποτελείται συνήθως από μονοκλωνικά IgG αντισώματα. Τα IgG είναι μονομερή, άρα η αντίδραση μπορεί να είναι λιγότερο έντονη. Σε πε
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